Saccharification of sunflower stalks using lignocellulases from a fungal consortium comprising Pholiota adiposa and Armillaria gemina.
Identifieur interne : 001B50 ( Main/Exploration ); précédent : 001B49; suivant : 001B51Saccharification of sunflower stalks using lignocellulases from a fungal consortium comprising Pholiota adiposa and Armillaria gemina.
Auteurs : Priyadharshini Ramachandran [Corée du Sud] ; Tae-Su Kim ; Saurabh Sudha Dhiman ; Jinglin Li ; Ji-Hyun Park ; Joon-Ho Choi ; Jae Young Kim ; Dongwook Kim ; Jung-Kul LeeSource :
- Bioprocess and biosystems engineering [ 1615-7605 ] ; 2015.
Descripteurs français
- KwdFr :
- MESH :
- composition chimique : Cellulase, Helianthus, Lignine, Tiges de plante.
- enzymologie : Armillaria, Pholiota.
- isolement et purification : Glucides.
- physiologie : Consortiums microbiens.
- synthèse chimique : Glucides.
English descriptors
- KwdEn :
- MESH :
- chemical , chemical synthesis : Carbohydrates.
- chemical , chemistry : Cellulase, Lignin.
- chemistry : Helianthus, Plant Stems.
- enzymology : Armillaria, Pholiota.
- chemical , isolation & purification : Carbohydrates.
- physiology : Microbial Consortia.
Abstract
Lignocellulases from Armillaria gemina and Pholiota adiposa are efficient in hydrolyzing aspen and poplar biomass, respectively. In the present study, lignocellulosic enzymes obtained from a fungal consortium comprising P. adiposa and A. gemina were used for the saccharification of sunflower stalks. Sunflower stalks were thermochemically pretreated using 2 % NaOH at 50 °C for 24 h. The saccharification process parameters including substrate concentration, enzyme loading, pH, and temperature were optimized using response surface methodology to improve the saccharification yield. The highest enzymatic hydrolysis (84.3 %) was obtained using the following conditions: enzyme loading 10 FPU/g-substrate, substrate 5.5 %, temperature 50 °C, and pH 4.5. The hydrolysis yield obtained using the enzymes from the fungal consortium was equivalent to that obtained using a mixture of commercial enzymes Celluclast and Novozyme β-glucosidase. Addition of up to 500 ppm of heavy metal ions (As, Cu, Fe, Mn, Ni, Pb, and Zn) during saccharification did not significantly affect the saccharification yield. Thus, the biomass grown for phytoremediation of heavy metals can be used for the production of reducing sugars followed by ethanol fermentation.
DOI: 10.1007/s00449-015-1406-7
PubMed: 25924967
Affiliations:
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sort="Dhiman, Saurabh Sudha" uniqKey="Dhiman S" first="Saurabh Sudha" last="Dhiman">Saurabh Sudha Dhiman</name></author><author><name sortKey="Li, Jinglin" sort="Li, Jinglin" uniqKey="Li J" first="Jinglin" last="Li">Jinglin Li</name></author><author><name sortKey="Park, Ji Hyun" sort="Park, Ji Hyun" uniqKey="Park J" first="Ji-Hyun" last="Park">Ji-Hyun Park</name></author><author><name sortKey="Choi, Joon Ho" sort="Choi, Joon Ho" uniqKey="Choi J" first="Joon-Ho" last="Choi">Joon-Ho Choi</name></author><author><name sortKey="Kim, Jae Young" sort="Kim, Jae Young" uniqKey="Kim J" first="Jae Young" last="Kim">Jae Young Kim</name></author><author><name sortKey="Kim, Dongwook" sort="Kim, Dongwook" uniqKey="Kim D" first="Dongwook" last="Kim">Dongwook Kim</name></author><author><name sortKey="Lee, Jung Kul" sort="Lee, Jung Kul" uniqKey="Lee J" first="Jung-Kul" last="Lee">Jung-Kul Lee</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date 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Hwayang-Dong, Gwangjin-Gu, Seoul, 143-701, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Chemical Engineering, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul, 143-701</wicri:regionArea><wicri:noRegion>143-701</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Tae Su" sort="Kim, Tae Su" uniqKey="Kim T" first="Tae-Su" last="Kim">Tae-Su Kim</name></author><author><name sortKey="Dhiman, Saurabh Sudha" sort="Dhiman, Saurabh Sudha" uniqKey="Dhiman S" first="Saurabh Sudha" last="Dhiman">Saurabh Sudha Dhiman</name></author><author><name sortKey="Li, Jinglin" sort="Li, Jinglin" uniqKey="Li J" first="Jinglin" last="Li">Jinglin Li</name></author><author><name sortKey="Park, Ji Hyun" sort="Park, Ji Hyun" uniqKey="Park J" first="Ji-Hyun" last="Park">Ji-Hyun Park</name></author><author><name sortKey="Choi, Joon Ho" sort="Choi, Joon Ho" uniqKey="Choi J" first="Joon-Ho" last="Choi">Joon-Ho Choi</name></author><author><name sortKey="Kim, Jae Young" sort="Kim, Jae Young" uniqKey="Kim J" first="Jae Young" last="Kim">Jae Young Kim</name></author><author><name sortKey="Kim, Dongwook" sort="Kim, Dongwook" uniqKey="Kim D" first="Dongwook" last="Kim">Dongwook Kim</name></author><author><name sortKey="Lee, Jung Kul" sort="Lee, Jung Kul" uniqKey="Lee J" first="Jung-Kul" last="Lee">Jung-Kul Lee</name></author></analytic><series><title level="j">Bioprocess and biosystems engineering</title><idno type="eISSN">1615-7605</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Armillaria (enzymology)</term><term>Carbohydrates (chemical synthesis)</term><term>Carbohydrates (isolation & purification)</term><term>Cellulase (chemistry)</term><term>Helianthus (chemistry)</term><term>Lignin (chemistry)</term><term>Microbial Consortia 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xml:lang="fr"><term>Cellulase</term><term>Helianthus</term><term>Lignine</term><term>Tiges de plante</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Armillaria</term><term>Pholiota</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Armillaria</term><term>Pholiota</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Carbohydrates</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Glucides</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Consortiums microbiens</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Microbial Consortia</term></keywords><keywords scheme="MESH" qualifier="synthèse chimique" xml:lang="fr"><term>Glucides</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Lignocellulases from Armillaria gemina and Pholiota adiposa are efficient in hydrolyzing aspen and poplar biomass, respectively. In the present study, lignocellulosic enzymes obtained from a fungal consortium comprising P. adiposa and A. gemina were used for the saccharification of sunflower stalks. Sunflower stalks were thermochemically pretreated using 2 % NaOH at 50 °C for 24 h. The saccharification process parameters including substrate concentration, enzyme loading, pH, and temperature were optimized using response surface methodology to improve the saccharification yield. The highest enzymatic hydrolysis (84.3 %) was obtained using the following conditions: enzyme loading 10 FPU/g-substrate, substrate 5.5 %, temperature 50 °C, and pH 4.5. The hydrolysis yield obtained using the enzymes from the fungal consortium was equivalent to that obtained using a mixture of commercial enzymes Celluclast and Novozyme β-glucosidase. Addition of up to 500 ppm of heavy metal ions (As, Cu, Fe, Mn, Ni, Pb, and Zn) during saccharification did not significantly affect the saccharification yield. Thus, the biomass grown for phytoremediation of heavy metals can be used for the production of reducing sugars followed by ethanol fermentation. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25924967</PMID><DateCompleted><Year>2016</Year><Month>05</Month><Day>09</Day></DateCompleted><DateRevised><Year>2015</Year><Month>08</Month><Day>14</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1615-7605</ISSN><JournalIssue CitedMedium="Internet"><Volume>38</Volume><Issue>9</Issue><PubDate><Year>2015</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Bioprocess and biosystems engineering</Title><ISOAbbreviation>Bioprocess Biosyst Eng</ISOAbbreviation></Journal><ArticleTitle>Saccharification of sunflower stalks using lignocellulases from a fungal consortium comprising Pholiota adiposa and Armillaria gemina.</ArticleTitle><Pagination><MedlinePgn>1645-53</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00449-015-1406-7</ELocationID><Abstract><AbstractText>Lignocellulases from Armillaria gemina and Pholiota adiposa are efficient in hydrolyzing aspen and poplar biomass, respectively. In the present study, lignocellulosic enzymes obtained from a fungal consortium comprising P. adiposa and A. gemina were used for the saccharification of sunflower stalks. Sunflower stalks were thermochemically pretreated using 2 % NaOH at 50 °C for 24 h. The saccharification process parameters including substrate concentration, enzyme loading, pH, and temperature were optimized using response surface methodology to improve the saccharification yield. The highest enzymatic hydrolysis (84.3 %) was obtained using the following conditions: enzyme loading 10 FPU/g-substrate, substrate 5.5 %, temperature 50 °C, and pH 4.5. The hydrolysis yield obtained using the enzymes from the fungal consortium was equivalent to that obtained using a mixture of commercial enzymes Celluclast and Novozyme β-glucosidase. Addition of up to 500 ppm of heavy metal ions (As, Cu, Fe, Mn, Ni, Pb, and Zn) during saccharification did not significantly affect the saccharification yield. Thus, the biomass grown for phytoremediation of heavy metals can be used for the production of reducing sugars followed by ethanol fermentation. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ramachandran</LastName><ForeName>Priyadharshini</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Department of Chemical Engineering, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul, 143-701, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Tae-Su</ForeName><Initials>TS</Initials></Author><Author ValidYN="Y"><LastName>Dhiman</LastName><ForeName>Saurabh Sudha</ForeName><Initials>SS</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Jinglin</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Park</LastName><ForeName>Ji-Hyun</ForeName><Initials>JH</Initials></Author><Author ValidYN="Y"><LastName>Choi</LastName><ForeName>Joon-Ho</ForeName><Initials>JH</Initials></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Jae Young</ForeName><Initials>JY</Initials></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Dongwook</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Jung-Kul</ForeName><Initials>JK</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>04</Month><Day>30</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Bioprocess Biosyst Eng</MedlineTA><NlmUniqueID>101088505</NlmUniqueID><ISSNLinking>1615-7591</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002241">Carbohydrates</NameOfSubstance></Chemical><Chemical><RegistryNumber>11132-73-3</RegistryNumber><NameOfSubstance UI="C036909">lignocellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.4</RegistryNumber><NameOfSubstance UI="D002480">Cellulase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D055392" MajorTopicYN="N">Armillaria</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002241" MajorTopicYN="N">Carbohydrates</DescriptorName><QualifierName UI="Q000138" MajorTopicYN="Y">chemical synthesis</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002480" 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MajorTopicYN="N">chemistry</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year><Month>05</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>04</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>5</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>5</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>5</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25924967</ArticleId><ArticleId IdType="doi">10.1007/s00449-015-1406-7</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Corée du Sud</li></country></list><tree><noCountry><name sortKey="Choi, Joon Ho" sort="Choi, Joon Ho" uniqKey="Choi J" first="Joon-Ho" last="Choi">Joon-Ho Choi</name><name sortKey="Dhiman, Saurabh Sudha" sort="Dhiman, Saurabh Sudha" uniqKey="Dhiman S" first="Saurabh Sudha" last="Dhiman">Saurabh Sudha Dhiman</name><name sortKey="Kim, Dongwook" sort="Kim, Dongwook" uniqKey="Kim D" first="Dongwook" last="Kim">Dongwook Kim</name><name sortKey="Kim, Jae Young" sort="Kim, Jae Young" uniqKey="Kim J" first="Jae Young" last="Kim">Jae Young Kim</name><name sortKey="Kim, Tae Su" sort="Kim, Tae Su" uniqKey="Kim T" first="Tae-Su" last="Kim">Tae-Su Kim</name><name sortKey="Lee, Jung Kul" sort="Lee, Jung Kul" uniqKey="Lee J" first="Jung-Kul" last="Lee">Jung-Kul Lee</name><name sortKey="Li, Jinglin" sort="Li, Jinglin" uniqKey="Li J" first="Jinglin" last="Li">Jinglin Li</name><name sortKey="Park, Ji Hyun" sort="Park, Ji Hyun" uniqKey="Park J" first="Ji-Hyun" last="Park">Ji-Hyun Park</name></noCountry><country name="Corée du Sud"><noRegion><name sortKey="Ramachandran, Priyadharshini" sort="Ramachandran, Priyadharshini" uniqKey="Ramachandran P" first="Priyadharshini" last="Ramachandran">Priyadharshini Ramachandran</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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